Seed germination behaviour of the narrow endemic Daphne arbuscula (Thymelaeaceae) compared to the more widespread Daphne cneorum

Abstract Diminished reproduction success in species with narrow distribution ranges might be one of the factors responsible for their limited dispersal and colonization abilities. We investigated here various aspects seed biology West Carpathian endemic Daphne arbuscula (Thymelaeaceae) compared it its more widespread relative D. cneorum . In both species, we (i) differences viability germination ability; (ii) between two observed fruit morphotype groups, (iii) effect cold stratification breaking dormancy enhance stored seeds. To determine viability, a tetrazolium test an imbibed cut were performed. Several tests gibberellic acid sequence warm stratification, using different temperatures durations, carried out. uncovered that seeds show significantly lower than seeds, but this difference is due to smaller-fruit morphotype; quality big-fruit are greater although latter not null level seems differ them; at 15°C 13 weeks, followed by either 0 or 5°C 28 4 weeks 15°C, break physiological allow majority (63%) germinate. recommend including morphotypes when collecting ex situ conservation reintroduction initiatives, maintain original genetic diversity species.